Features and Benefits

Compatible with high-throughput handling systems.

General description

L-Arginine (Arg) is a proteogenic, semi-essential amino acid: healthy humans can synthesize L-Arginine using L-Glutamine as a building block; however, premature infants are unable to produce Arg and additional supplementation is required for proper growth and development. Arginine plays pivotal roles in biochemical pathways such as the urea cycle and the biosynthesis of nitric oxide. Arginine and Ammonia concentrations are elevated in patients having a mutation in their ARG1 genes. The mutation causes lower arginase activities – a condition that is known as Argininemia. Arginine has also been advertised as a supplement due to its role in the synthesis of nitric oxide, which helps in vasodilation processes.

Principle

The L-Arginine Assay Kit provides a quick, specific, and easy method for the measurement of total L-arginine concentrations in a wide variety of samples. In this enzyme-based assay, L-arginine is converted into a series of intermediates, which will further react with a probe producing a stable colorimetric signal at A450 nm. The kit is simple to use, sensitive and high-throughput adaptable and can detect as low as 1 nmol/well of L-arginine in biological samples.

Suitability

Suitable for the measurement of L-arginine in biological samples (serum, etc) and beverages (orange juice, etc), the analysis of the relationship of L-arginine intake and nitric oxide production and the analysis of the urea cycle.